Effects of Plant Growth Regulators on Shoot Multiplication and Root Induction of Cassava Varieties Culture in Vitro

摘要

A study on propagation of three superior cassava Manihot esculenta( Crant) varieties i.e.Darul Hidayah, Malang-6 and Adira-4 through tissue culture technique was conducted at theTissue Culture Lab of SEAMEO BIOTROP, Bogor. The objective of the experiment was tostudy effect of plant growth regulators on Manihot esculenta propagation, which can be used in cassavamicropropagation protocol. Plant materials used were auxiliary shoots of a stem node. Theexperiment consisted of (i) shoot multiplication, (ii) roots induction, and (iii) acclimatization.The multiple shoot regeneration was observed by using Murashige & Skoog (MS) basal mediasupplemented with 0, 0.1, 1.0 and 5.0 mg/L of benzylaminopurine (BAP) combined with 0.0,0.1 and 1.0mg/L of thidiazuron. The root induction was observed by using 0.1 and 1.0mg/Lof IBA and NAA. The resulting plantlets were transplanted into plastic polybags containingsoil mixed with organic fertilizer (1:1) covered with plastic sheets and transferred to agreenhouse. The result of the study showed that the highest number of shoots for DarulHidayah, Malang-6 and Adira-4 varieties were 4.93 shoots treated with BAP 1 mg/L +thidiazuron 0.1 mg/L, 4.20 shoots at BAP media of 1 mg/L, and 7.20 shoots at the mediaof BAP 1 mg/L + thidiazuron 0.1 mg/L respectively. The highest number of nodes producedwas 2.9 nodes for Darul Hidayah at BAP 5 mg/L, 5.13 nodes for Malang-6 at BAP 0.1 mg/L,and 6.18 nodes forAdira-4 at BAP 5mg/L + thidiazuron 1mg/L. The utilization of auxin IAAor NAA could induce and accelerate the growth of roots which finally could increase thesuccess of acclimatization process. With an average of four multiplication factors of eachculture period, the potency of each cassava shoot propagated through tissue culture couldproduce around 37 000 plants/year.